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Correlation between JAK2 activity and phosphorylation of STAT1 Ser727. BAC1.2F5 cells were treated with IFN-γ, LPS, or GM-CSF for 20 min. JAK2 activation was determined after immunoprecipitation from cellular extracts and Western blots with phosphotyrosine-specific monoclonal antibody Py20. Phosphorylation of STAT1 Ser727 was determined in the same extracts by Western blotting.
