Figure 4.

Production of IL-4 and histamine by splenic non-B, non-T cells from Nb-inoculated mice. (A) Freshly prepared splenic non-B, non-T cells from each group of mice described in Fig. 3 were analyzed for expression of FcɛR and c-kit by flow cytometry. The percentages shown represent the proportion of FcɛR⁺/c-kit⁻ cells. (B) Splenic non-B, non-T cells (2 × 10⁵/0.2 ml per well) from each group of mice were stimulated with medium alone or IL-3 and/or IL-18 without FcɛR cross-linking for 24 h. Supernatants were analyzed for production of IL-4 and histamine. Results are geometric means ± SEM.