Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1985 Jun;49(6):1448–1454. doi: 10.1128/aem.49.6.1448-1454.1985

Rate of Bacterial Mortality in Aquatic Environments

Pierre Servais 1, Gilles Billen 1,*, Jose Vives Rego 1
PMCID: PMC241745  PMID: 16346811

Abstract

A method is proposed which provides a minimum estimate of the rate of bacterial mortality in growing natural populations of planktonic bacteria. This estimate is given by the rate of decrease of radioactivity from the DNA of a [3H]thymidine-labeled natural assemblage of bacteria after all added thymidine has been exhausted from the medium. Results obtained from river water, estuarine water, and seawater show overall bacterial mortality rates in the range 0.010 to 0.030 h−1, in good agreement with the range of growth rates measured in the same environments. Use of selective filtration through Nuclepore filters (pore size, 2 μm) allowed us to determine the contribution of microzooplankton grazing to overall bacterial mortality. Grazing rates estimated by this method ranged from 0 to 0.02 h−1.

Full text

PDF
1448

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell R. T., Ahlgren G. M., Ahlgren I. Estimating Bacterioplankton Production by Measuring [H]thymidine Incorporation in a Eutrophic Swedish Lake. Appl Environ Microbiol. 1983 Jun;45(6):1709–1721. doi: 10.1128/aem.45.6.1709-1721.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Casida L. E. Bacterial Predators of Micrococcus luteus in Soil. Appl Environ Microbiol. 1980 May;39(5):1035–1041. doi: 10.1128/aem.39.5.1035-1041.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ducklow H. W., Kirchman D. L., Rowe G. T. Production and vertical flux of attached bacteria in the hudson river plume of the new york bight as studied with floating sediment traps. Appl Environ Microbiol. 1982 Apr;43(4):769–776. doi: 10.1128/aem.43.4.769-776.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fuhrman J. A., Azam F. Bacterioplankton secondary production estimates for coastal waters of british columbia, antarctica, and california. Appl Environ Microbiol. 1980 Jun;39(6):1085–1095. doi: 10.1128/aem.39.6.1085-1095.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HINSHELWOOD C. Decline and death of bacterial populations. Nature. 1951 Apr 28;167(4252):666–669. doi: 10.1038/167666a0. [DOI] [PubMed] [Google Scholar]
  6. Hagström A., Larsson U., Hörstedt P., Normark S. Frequency of dividing cells, a new approach to the determination of bacterial growth rates in aquatic environments. Appl Environ Microbiol. 1979 May;37(5):805–812. doi: 10.1128/aem.37.5.805-812.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KOCH A. L. Death of bacteria in growing culture. J Bacteriol. 1959 May;77(5):623–629. doi: 10.1128/jb.77.5.623-629.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Karl D. M. Selected nucleic Acid precursors in studies of aquatic microbial ecology. Appl Environ Microbiol. 1982 Oct;44(4):891–902. doi: 10.1128/aem.44.4.891-902.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Newell S. Y., Christian R. R. Frequency of dividing cells as an estimator of bacterial productivity. Appl Environ Microbiol. 1981 Jul;42(1):23–31. doi: 10.1128/aem.42.1.23-31.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. POSTGATE J. R., CRUMPTON J. E., HUNTER J. R. The measurement of bacterial viabilities by slide culture. J Gen Microbiol. 1961 Jan;24:15–24. doi: 10.1099/00221287-24-1-15. [DOI] [PubMed] [Google Scholar]
  12. POSTGATE J. R., HUNTER J. R. The survival of starved bacteria. J Gen Microbiol. 1962 Oct;29:233–263. doi: 10.1099/00221287-29-2-233. [DOI] [PubMed] [Google Scholar]
  13. Payne W. J. Energy yields and growth of heterotrophs. Annu Rev Microbiol. 1970;24:17–52. doi: 10.1146/annurev.mi.24.100170.000313. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES