Figure 6.

Localization of β-gal/18D6 fusion, M31, and ATRX proteins on mouse (F9/18D6) metaphase chromosomes. Immunostaining of F9/18D6 metaphase chromosomes with antibody against β-gal (images on the far left and red in merged images), together with antibodies against M31 (Upper, second from left) and ATRX (Lower, second from left and green in merged images), is shown. DNA was counterstained with DAPI (second from right and blue in merged images). Note the intense DAPI staining at the pericentromeric heterochromatin of the mouse chromosomes. Colocalized signals appear as white.