Skip to main content
. 1999 Nov 23;96(24):13989–13994. doi: 10.1073/pnas.96.24.13989

Figure 1.

Figure 1

(A) Effects of oligos on the expression of the RIα subunit of PKA in LS174T and MDA-MB-468 cells. Cultured cells were treated with Oligo AS or ASM at various concentrations for 24 h. Identical amounts of total protein (50 μg) were fractionated by SDS/PAGE and analyzed by Western blotting. For LS174T cells, at the concentrations of 1 and 10 μM, Oligo AS inhibited RIα subunit expression by about 80%, with no significant dose–response being observed at higher concentrations. For MDA-MB-468 cells, a significant dose–response relationship was observed. At the highest concentration, Oligo AS inhibited the RIα subunit expression by about 75%. In contrast, Oligo ASM had no effect on RIα subunit expression for either cell line. (B) Effects of oligos on the expression of the RIα subunit of PKA in LS174T and MDA-MB-468 tumor xenografts. Animals with LS174T xenografts were treated with oral administration of Oligo AS or ASM for 1 week. Animals with MDA-MB-468 xenografts were treated with oral administration of Oligo AS or ASM for 6 weeks. Identical amounts of total protein (100 μg) from the tumor homogenates were used in Western blot analysis. A dose-dependent inhibition of the RIα subunit expression was observed in both tumor models. At 10 mg/kg, Oligo AS inhibited RIα subunit expression by about 80%. In contrast, Oligo ASM had no effect.