Figure 3.

Microarray expression profiling of 5-Aza-CdR and IFN-treated HT29 cells. HT29 cells were treated with 500 nM 5-Aza-CdR, 1 × 10⁵ units/ml IFN-α2a, or 5 × 10² units/ml IFN-γ. RNA was harvested 9 days (5-Aza-CdR) or 4 days (96 hr) (IFN-α or -γ) after treatment and used to generate probes for microarray analysis. Shown in the figure is a representative section of the microarray after hybridization with Cy-5-labeled cDNAs from 5-Aza-CdR-, IFN-α-, or IFN-γ-treated cells and Cy-3-labeled cDNAs from control cells. Four genes up-regulated by 5-Aza-CdR treatment are on the displayed grid. They are IFN-α-inducible protein 6–16 (row 4, column 9), expressed sequence tag (EST) Hs.109309 (8, 7), EST Hs.165240 (9, 14), and human mRNA for Stac (9, 16).