Table 1.
Genes up-regulated by 5-Aza-CdR treatment of HT29 cells*
| 5-Aza-CdR-induced gene† | Unigene number or image ID | Regulation by IFN-α‡ | Regulation by IFN-γ‡ |
|---|---|---|---|
| Human mRNA for Stac | Hs. 56045 | + | + |
| IFN-induced protein 56 | Hs. 20315 | + | − |
| IFNα-inducible protein 27 | Hs. 2867 | + | + |
| IFN-induced 17-kDa protein | IID 149319 | + | + |
| EST | Hs. 6166 | + | − |
| EST | Hs. 165240 | + | + |
| Myxovirus resistance gene 2 | Hs. 926 | + | − |
| Purinergic receptor P2Y5 | Hs. 189999 | + | + |
| EST | Hs. 109309 | + | − |
| CpG island DNA fragment | No match§ | + | + |
| TGF-β superfamily member MIC-1 | Hs. 116577 | + | + |
| IFN-induced protein IFI-6-16 | Hs. 21205 | + | − |
| EST | Hs. 47783 | + | + |
| MHC class I | Hs. 77961 | + | + |
| Midkine | Hs. 82045 | − | + |
| Myxovirus resistance gene 1 | Hs. 76391 | + | − |
| 2′-5′-Oligoadenylate synthetase 3 | No match¶ | + | + |
| Nuclear antigen SP100 | Hs. 77617 | + | − |
| IFN-inducible protein 10 | Hs. 2248 | − | + |
Bold type indicates previously identified IFN-responsive genes.
Genes induced in HT29 cells by treatment with 500 nM 5-Aza-CdR at day 9 that were up-regulated by greater than 2 SD above the mean expression ratio for all genes on the microarray (see Fig. 2B), listed in descending order.
† The identity of each gene was verified by DNA sequencing and blast analysis (33).
‡ The expression of the genes induced by 5-Aza-CdR (column 1) was measured by microarray analysis after treatment of HT29 cells with 1 × 105 units/ml IFN-α2a or 5 × 102 units/ml IFN-γ for 10, 24, and 96 hr. Expression was scored as induced if the gene was up-regulated at any time point.
§ Identical to accession numbers Z61029 and Z61030 (100% identity over 115 bases) in the nonredundant Genbank database.
¶ Identical to accession number NM_006187 (100% identity over 366 bases) in the nonredundant Genbank database.