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. 1999 Nov 23;96(24):14025–14030. doi: 10.1073/pnas.96.24.14025

Figure 3.

Figure 3

RNase protection to quantify expression of exons 1B and 2A. (A) Specific protected fragments of 206 bp (exon 2A/3) and 135 bp (exon 1B/3) were generated by simultaneous hybridization of two probes with mouse, but not yeast, RNA. (B) The signal generated is linearly related to the amount of RNA for both probes under these conditions, allowing quantitative analysis. (C) Amount of each transcript present (in arbitrary units) in 30 μg of total RNA from four different tissues. H, heart; B, brain; M, muscle; K, kidney.