Figure 5.

HCC mediated by DLC1 loss requires RhoA and is sensitized to Rho inhibitors. (A) Hepatoma cell line BNL is infected by retroviruses expressing control shRNA (control) or three RhoA shRNAs and immunoblotted for RhoA protein. Tubulin serves as a loading control. Numbers denote relative protein abundance. (B) RhoA shRNAs selectively suppress tumors harboring low levels of DLC1. Relative tumor volume is the last measurement in Figure S6A-D. (*) P < 0.05; (**) P < 0.0005; (#) P > 0.05. DLC1 mRNA level is measured by qRT–PCR and normalized to control liver progenitor cells. (C,D) Loss of DLC1 sensitizes liver cells to ROCK kinase inhibitors Y27632 and Fasudil. Error bars denote SD (n = 3).