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Gain of transformation response in P− cells expressing antisense Pdcd4. Cell lines were exposed to the solvent control 0.1% DMSO or to 10 ng/ml (16 nM) of TPA for 14 days in soft agar. Dishes were stained with 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride and photographed at 25× magnification. Examples representative of several experiments are shown.
