Table 1.

Complex Assembly and Kinetic Parameters for Cre-Lox Recombination Reactions.^a

		Complex assembly			Substrate turnover
Cre protein(s)	DNA substrates	extentb (%)	S0.5 (nM)	Hill (α)	extentb (%)	A	k1×103 (sec−1)	k2×103 (sec−1)	t1/2 (sec)
CreWT	LoxP x LoxP	47 ± 12	71±5	2.8±0.1	60 ± 6c	0.42±0.02	4.5±1.3	32±3	48
	LoxM7 x LoM7	NRd at 2400 nM complex
CreALSHG	LoxM7 x LoxM7	49 ± 12	150±16	3.7±0.5
	LoxP x LoxP	38 ± 84	313±10	3.6±0.9	20 ± 42	0.66±0.03	1.1±0.2	9.7±0.1	300
CreAAF	LoxP x LoxP	45 ± 44	154±17	2.1±0.2	45 ± 32	0.84±0.01	1.5±0.7	22±4	346
CreWT+	LoxPM7 x LoxPM7	54 ± 54	132±17	3.9±0.7	58 ± 12	0.59±0.07	5.3±0.1	23±1	67
CreALSHG	LoxM7P x LoxPM7	54 ± 22	144±18	4.3±0.4	56 ± 22	0.63±0.03	1.4±0.1	14±2	198
CreAAF+	LoxP x LoxP	21 ± 83	266±22	4.8±2.1
CreALSHG
CreAA+	LoxM7P x LoxM7P	53 ± 55	179±19	3.6±0.6	65 ± 74	0.51±0.13	4.7±0.6	27±6	56
ALSHG-F	LoxM7P x LoxPM7	<3% turnover at 1200 nM complex
	LoxP x LoxM7	9 ± 23	178±9	5.9±1.3	10 ± 82	0.73±0.03	0.93±0.03	33±2	402
	LoxM7 x LoxM7	NR at 360 nM complex
	LoxP x LoxP	reaction in qualitative assay comparable to LoxM7P x LoxM7P
ALSHG-F	LoxM7P x LoxM7P	NR at 360 nM complex
	LoxM7P x LoxPM7	NR at 360 nM complex
	LoxP x LoxM7	NR at 360 nM complex
	LoxM7 x LoxM7	NR at 360 nM complex
	LoxP x LoxP	NR at 360 nM complex
CreAA	LoxM7P x LoxM7P	NR at 360 nM complex
	LoxM7P x LoxPM7	NR at 360 nM complex
	LoxP x LoxM7	NR at 360 nM complex
	LoxM7 x LoxM7	NR at 360 nM complex
	LoxP x LoxP	some products in qualitative assay at > 240 nM complexe

^aComplex assembly and kinetic parameters were calculated as described in METHODS AND MATERIALS, and are given as the averaged values from independent fits of replicate experiments ± S.D. (n > 2) or S.E (n=2).

^bsuperscripts denote number of independent replicates.

^cFrom reference ⁴⁴

^dNR = no detectable reaction in the qualitative assay.

^eFigure 5a (middle panel).