Table 1.

Regulation of a translational hcnA′-′lacZ fusion on pME3219 by the gacS/gacA and anr genes in P. fluorescens

Host*	Relevant genotype	β-galactosidase activity, (Miller units)†
CHA0	wild type	25,800  ± 3,100
CHA89	gacA∷Km	440  ± 120
CHA510	gacS∷Tn5	490  ± 100
CHA21	anr∷Ω-Km	5  ± 0.5

^*Each host strain carried pME3219. β-galactosidase activity (Miller units) was determined when cells were mildly oxygen-limited at an OD₆₀₀ of 2.0–2.5. Under these conditions, the hcnA promoter is partially induced. 

^†Means of three or more independent experiments (± standard deviation).