Table 3.

Strict correlation between GacA- and RsmA-dependent regulation in P. fluorescens

Strain/plasmid*	β-galactosidase activity†		RsmA repression factor	GacA induction factor‡
	+ pME6001, vector control	+ pME6073, rsmA++
CHA0/pME3219	12,400  ± 1,000	2,600  ± 500	4.8	59
CHA0/pME6530	27,200  ± 1,500	6,400  ± 450	4.0	30
CHA0/pME6533	14,400  ± 700	2,600  ± 80	5.5	33
CHA0/pME6536	46,800  ± 1,500	36,000  ± 2,300	1.3	1.5
CHA0/pME6537	39,300  ± 1,900	29,700  ± 3,000	1.3	1.4
CHA0/pME6538	4,300  ± 200	830  ± 40	5.2	45
CHA0/pME6539	29,400  ± 900	20,000  ± 1,200	1.5	1.1
CHA0/pME6541	12,400  ± 500	2,100  ± 140	6.0	35
CHA0/pME6521	7,800  ± 510	7,600  ± 290	1.0	1.3
CHA0/pME6544	3,400  ± 70	1,000  ± 30	3.4	46

^*The hcnA′-′lacZ fusion constructs (Figs. 1 and 4) were tested in the presence (pME6073) or absence (pME6001) of overexpressed RsmA. Cells were grown in 20 ml of nutrient yeast broth with gentamicin (10 μg/ml) to an OD₆₀₀ of 2.0–2.5. 

^† β-galactosidase activities (Miller units) were determined in triplicate; mean values ± standard deviation are given. 

^‡Values are from Fig. 4.