Figure 5.

G_o −/− mice have smaller glomeruli in the posterior AOB. (a) P15 AOB parasagittal sections were stained with anti-synaptophysin or anti-MAP2 antibodies. The following areas of the AOB are indicated: vomeronasal nerve layer (VN), glomerular layer (GL), mitral and tufted cell layer (MT), anterior (Ant), posterior (Post), and the border of the two AOB lobes (arrowheads). In the G_o −/− sections, the posterior GL is reduced in area, but the staining characteristics of different layers are normal. (Bar = 100 μm.) (b) The area of the anterior and posterior AOB-GL revealed by anti-synaptophysin staining was quantitated in mid AOB sections from P15 mice. The area of the posterior AOB-GL is significantly decreased in the G_o −/− mice (*, P ≤ 0.05, Student’s two-tailed t test). The data are means ± SEM from 5–8 animals in each group. (c) Glomerular structures are visualized by double staining of P15 posterior AOB parasagittal sections with anti-synaptophysin antibody (red) and 4′,6-diamidino-2-phenylindole (blue). In the control samples, glomeruli are diffuse and large. In the G_o −/− samples, the posterior GL layer has glomeruli of reduced area, but the total number is similar to that of controls. (Bar is 50 μm.)