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. 1999 Nov 23;96(24):14165–14170. doi: 10.1073/pnas.96.24.14165

Figure 1.

Figure 1

Analysis of transgenic tobacco plants with reduced cAPX protein. (A) A protein blot of transgenic and control plants showing that the level of cAPX protein is reduced dramatically in transgenic plants that express antisense RNA for cAPX compared with control plants. The level of PR-1 is shown not to be induced in untreated transgenic or control plants. As a positive control we used application of SA (1 mM; ref. 23). This treatment induced the expression of PR-1 in control plants (SA). The level of HSP70 and RbcL was used to demonstrate equal loading of protein. Analysis was performed on two individual transgenic plants and two individual control plants. (B) Hyperresponsiveness of transgenic plants with reduced levels of cAPX to oxidative stress induced by paraquat. The graph shows enhanced cell death in antisense-APX plants exposed to different concentrations of paraquat compared with control plants.