Figure 4.

Antioxidants and an inhibitor of ROI generation during the HR suppress bacteria-induced cell death in transgenic plants with reduced cAPX protein. (A) Inhibition of HR cell death by a mixture of antioxidants (ASC+GSH; 20 mM reduced ASC and 10 mM reduced GSH) or by CAT (1,000 units/ml). CAT or antioxidants were infiltrated into leaves of control and transgenic plants together with bacteria (OD₆₀₀ = 0.1). Twelve hours after inoculation, leaves were sampled and analyzed for cell death. (B) Inhibition of HR cell death by an inhibitor of the NAD(P)H oxidase complex (DPI; 10 μM). DPI was infiltrated into leaves of control and transgenic plants together with bacteria (OD₆₀₀ = 0.1). Twelve hours after inoculation leaves were sampled and analyzed for cell death. (C) Inhibition of bacteria-induced nuclear DNA fragmentation in transgenic plants with reduced cAPX protein by DPI. Total DNA was extracted from duplicate leaf samples that were obtained as described in B. DNA fragmentation was assayed by FIGE.