Figure 1.
In situ hybridization of LF mRNA in the ovariectomized wild-type mouse uterus (A) after exposure to oil (control), E2 (10 μg/kg), and/or ICI (20 mg/kg), and (B) after exposure to 4-OH-E2 (10 μg/kg) and 4-OH-E2 plus ICI or kepone (15 mg/kg). Bright- and dark-field photomicrographs of uterine sections are shown. (×100.) (A a and b) Oil (vehicle). (c and d) E2. (e and f) ICI + E2. (g and h) ICI. (B i and j) 4-OH-E2. (k and l) ICI + 4-OH-E2. (m and n) Kepone. No positive signals were observed when sections were hybridized with the sense probe (data not shown). LE, luminal epithelium; GE, glandular epithelium; S, stroma. These experiments were repeated three times with three mice in each group, and similar results were obtained. (C) Northern blot analysis of LF and rpL7 mRNAs. Effects of ICI {1 mg/kg [ICI (1)] or 20 mg/kg [ICI (20)]} on E2- or 4-OH-E2-induced uterine LF mRNA levels in wild-type mice are shown. The pooled total RNA (6 μg) obtained from three to four mice in each group was analyzed. These experiments were repeated twice with independent RNA samples and similar results were obtained.