Table 1.

Effects of E₂, 4-OH-E₂, or kepone on uterine LF mRNA levels in ovariectomized wild-type mice

Treatments	Levels of mRNA, fg/μg total RNA	mRNA copies, molecules/μg total RNA	Fold increase
Oil	21.8	4,800	1.0
E2	2126.4	470,000	97.6
Kepone	426.8	94,000	19.6
4-OH-E2	1857.4	400,000	85.2

Mice were given two injections at 6-h intervals of oil (control, 0.1 ml/mouse), E₂ (10 μg/kg), 4-OH-E₂ (10 μg/kg), or kepone (15 mg/kg), and were killed 6 h after the last injection. In each treatment group the pooled uterine total RNA from three to four mice was subjected to RT-PCR as described. Values were derived from the zero equivalence point of the logarithmic plot as shown in Fig. 2, and fold increases were calculated with respect to oil value. Values represent the average of two independent experiments.