Table 2.
Levels of uterine LF mRNA in ovariectomized ERKO mice after treatment with various agents
| Treatments | Levels of mRNA, fg/μg total RNA | mRNA copies, molecules/μg total RNA | Fold increase |
|---|---|---|---|
| Oil (n = 4) | 18.9 ± 1.0a | 4,100 ± 200a | 1.0 |
| E2(n = 4) | 27.0 ± 2.7a | 5,900 ± 200a | 1.4 |
| 4-OH-E2(n = 5) | 1126.5 ± 40.2b | 240,000 ± 10,000b | 59.6 |
| 4-OH-E2 + ICI (n = 3) | 1249.7 ± 40.5b | 280,000 ± 10,000b | 66.0 |
| 4-OH-E2 + E2(n = 3) | 1152.0 ± 65.9b | 250,000 ± 10,000b | 60.9 |
| Kepone (n = 4) | 360.3 ± 24.0c | 84,000 ± 3,000c | 19.0 |
| Kepone + ICI (n = 3) | 369.7 ± 22.8c | 82,000 ± 2,000c | 19.5 |
Mice were given two injections at 6-h intervals of oil (control, 0.1 ml/mouse), E2 (10 μg/kg), 4-OH-E2 (10 μg/kg), kepone (15 mg/kg), 4-OH-E2 + ICI (20 mg/kg), 4-OH-E2 + E2, or kepone + ICI. Mice were killed 6 h after the last injection. “n” denotes the number of replicates. For each replicate the pooled uterine total RNA from three to four mice was subjected to RT-PCR as described. Values were derived from the zero equivalence point of the logarithmic plot as shown in Fig. 4, and fold increases were calculated with respect to oil value. Values with different superscript letters are statistically different (p < 0.05, ANOVA followed by Newman–Keul’s multiple range test).