Correlation of the
log(kcat/Km)
and the ratio of
ln(kH/kT)/ln(kD/kT)
for site-directed mutants of alcohol dehydrogenase. Point A, Val-203
→ Gly; point B, Val-203 → Ala:Phe-93 → Trp; point C, Val-203 →
Ala; point D, Val-203 → Leu; point E, Phe-93 → Trp; point F, Leu-57
→ Phe. Tunneling is indicated when the ratio of
ln(kH/kT) to
ln(kD/kT) is >3.3
(8).