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Purification of the CPS from P. furiosus
| Purification step | Total protein, mg | Total activity, units | Specific activity, units/mg | Yield, % | Purification rate |
|---|---|---|---|---|---|
| Crude extract | 4,400 | 8,500 | 2 | 100 | 1 |
| (NH4)2SO4 fractionation | 3,400 | 8,000 | 2.5 | 94 | 1.25 |
| DEAE-Sepharose | 800 | 8,300 | 10 | 97 | 5 |
| Blue Sepharose | 150 | 8,200 | 55 | 96 | 30 |
| Sephadex G200 | 20 | 4,400 | 220 | 52 | 110 |
| Superose P12 | 3.5 | 2,100 | 600 | 25 | 300 |
| Mono-Q | 0.75 | 500 | 700 | 6 | 350 |
