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. 1997 Nov 25;94(24):12829–12832. doi: 10.1073/pnas.94.24.12829

Figure 2.

Figure 2

IL-1 induces complex formation of IL-1RI and IL-1RAcP. (A) Immunoblot analysis to detect IL-1RAcP precipitated from protein extract of 293IL-1RI cells (10-cm dish) with rabbit preimmune serum or antiserum raised to the extracellular domain of human IL-1RAcP. (B) IL-1-dependent coprecipitation of IL-1RI and IL-1RAcP. Immunoblot analysis was performed to detect IL-1RI in the immunocomplex precipitated with antiserum to the extracellular domain of IL-1RAcP from IL-1-treated (100 ng/ml for 5 min) or untreated 293IL-1RI cells. Rabbit preimmune serum was used as control for immunoprecipitation. (C) Interaction of transiently expressed IL-1RAcP and IL-1RI. 293IL-1RI cells were transfected for 20 h with vector alone, expression plasmids for Flag epitope-tagged IL-1RAcP, or AcP(1–403) and treated with IL-1 (100 ng/ml) for 5 min or left untreated. The immunocomplexes precipitated with anti-Flag antibody were subjected to immunoblot analysis to detect coprecipitating IL-1RI.