Figure 2.

Binding specificity. (A) Immunoprecipitation of radioactively labeled XAP1 or RNA transcribed from the unselected libraries G²QCL or G³QCL with preimmune serum or anti-NES antibodies, analyzed on a denaturing acrylamide gel (label at bottom is free GTP). (B) Immunoprecipitation of radioactively labeled XAP1 with anti-NES antibodies that had been preincubated with either buffer (−), NES peptide (NES), or control peptide (control) and analyzed on a denaturing acrylamide gel. (C) Immunoprecipitation of radioactively labeled XAPs or variants with anti-NES antibodies analyzed on a denaturing acrylamide gel. (D) Binding specificity of anti-NES antibodies analyzed by Western blotting of recombinant Rev wild type and Rev M10.