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. 1997 Nov 25;94(24):12845–12850. doi: 10.1073/pnas.94.24.12845

Figure 3.

Figure 3

Phasing analysis of DNA bending by the HMG domain of LEF-1. (A) Schematic structures of the protein/DNA complexes in which the protein-induced DNA bend is in phase with the intrinsic DNA bend (spacer length of 9) or out of phase (spacer length of 3). The filled circle indicates the LEF-1-binding site, which is separated by DNA of variable lengths (70–81 nt) from six A tracts. (B) Electrophoretic mobility-shift assay of purified LEF-HMG domain peptide (3 ng) bound to phased DNA probes. The numbers below indicate nucleotides inserted between the LEF-1-binding site and the A tract DNA. (C) Relative mobilities of protein/DNA complexes as a function of the spacer length (solid line). The broken line shows the variation in the mobilities of the free DNA probes.