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Gα binding by RGS4 mutants. (A) Coomassie stain of 5 μg of bacterially expressed RGS4 proteins purified by Ni2+-nitrilotriacetic acid chromatography. (B) Binding of RGS4 mutant proteins to GDP, GDP-AlF4−, or GTPγS-activated Giα1. The input amount of Giα1 and the entire amount of protein eluted from each binding reaction were detected by immunoblotting.
