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SDS/PAGE of different steps in DXP synthase purification. Lanes 1 and 7, Combithek size markers (Boehringer Mannheim). Lane 2, Cell-free extract of strain JM109/pBM20dxs without IPTG; lane 3, same plus IPTG; lane 4, after ammonium sulfate precipitation (40% saturation); lane 5, after anion-exchange chromatography on Q Sepharose HP; lane 6, after anion-exchange chromatography on DEAE-650S tentacle column. DXS denotes the protein band containing DXP synthase.
