Table 1.

DXP synthase activities of cell-free extracts of E. coli

Source of enzyme	Specific activity, nmol⋅min−1 per mg protein
E. coli LJ110	0.4
E. coli JM109/pUCBM20dxs −IPTG	12.2
E. coli JM109/pUCBM20dxs +IPTG	51.6
DXP synthase (DEAE-650S fraction)	850

Extracts were of wild-type E. coli LJ110, of recombinant strain JM109/pUCBM20dxs after growth of cells in the absence (−IPTG) or presence (+IPTG) of inducer, and of the enriched DXP synthase preparation after the final DEAE-650S tentacle anion-exchange chromatography.