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CNS-Specific KO of SF-1 Alters the Distribution of ERα-Positive Neurons in the Mediobasal Hypothalamus
Immunohistochemistry was performed and ERα-positive cells were counted in 80-μm wide columns as described in Materials and Methods. In WT mice, most immunoreactive cells were located laterally (columns 5–7, 400–560 μm from the third ventricle). In CNS-specific SF-1 KO mice, ERα-immunoreactive cells were located significantly more medially (P < 0.01, Genotype × Column ANOVA).
