Figure 7.

Locomotor Activity of WT and CNS-Specific SF-1 KO Mice in Habitual and Novel Settings

A, Locomotor activity in a habitual setting was measured with a running wheel as described in Materials and Methods for adult WT and CNS-specific SF-1 KO mice. The 0–12 on the x-axis indicates daytime (light) and 12–24 indicates nighttime (dark). Zeitgeber time (ZT) is plotted on the x-axis, and the average number of wheel turns is plotted on the y-axis. Vertical bars indicate sem. Wheel-running activity was significantly lower in CNS-specific SF-1 KO mice than in WT controls. B, Locomotor activity of WT and CNS-specific SF-1 KO mice also measured in a novel environment. CNS-specific SF-1 KO mice showed significantly higher numbers of beam breaks/min over a 15-min period. The data (A and B) were expressed as mean ± sem and analyzed by repeated measures two-way ANOVA followed by Bonferroni’s post hoc correction. (P < 0.05, genotype × time). C, Total movement of WT and CNS-specific SF-1 KO mice situated in a novel environment is shown as total distance (cm) of movement vs. time. CNS-specific SF-1 KO mice showed significantly higher movement than WT controls (*, P < 0.01).