Figure 4.

LPS Induces Loss of Hepatic GHR Protein without Change in Levels of GHR L2 mRNA in C3H/HeJ Mice

A, As detailed in Materials and Methods, mice were pretreated with LPS or PBS for the indicated durations. After killing, livers were homogenized and equal aliquots of cytoplasmic extract were immunoblotted to detect GHR. B, Densitometric analysis of GHR abundance from the blot in A. Data are plotted as the percentage of GHR remaining after LPS treatment at each time point relative to the control (mice treated with PBS for the same duration as the LPS treatment). Data are representative of three independent experiments. C, GHR L2 mRNA abundance. Mice treated with LPS for the indicated durations were killed, and liver GHR mRNA levels were determined by RT-PCR, as in Materials and Methods. Expression of the housekeeping gene GAPDH was used as an internal control to normalize results (n = 3–6; mean and range) depicted relative to the expression in control (PBS treated) animals.