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The PC1/3 Proximal Promoter Region
A 212-bp fragment of the PC1/3 promoter was cloned into the pGL3 basic plasmid and used for luciferase reporter assays. Two putative STAT3 binding sites (underlined) and E-box motifs (bold) are indicated as site 1 and site 2. Gray boxes indicate primers used in the ChIP and EMSA assays.
