Skip to main content
. 2008 Mar 20;22(6):1438–1448. doi: 10.1210/me.2008-0010

Figure 2.

Figure 2

PC1/3 mRNA Levels in the Hypothalamus Respond Normally to Signals of Energy Availability in WT Mice But Not in N2KO Mice

Whole hypothalamic RNA was isolated from WT and N2KO animals that were given ad libitum access to food (Ad lib), food deprived for 24 h (Deprived), or food deprived for 24 h and exposed to leptin for 2 h (Leptin). Relative expression levels of PC1/3 RNA, which were measured using qRT-PCR and normalized to β-actin, are shown. The results are expressed as mean ± se; *, P < 0.05; **, P < 0.01 to WT ad lib except where indicated; the bar indicates significance between genotypes; #, P < 0.05 to N2KO ad lib (n = 6 animals per condition).