Leptin Stimulation of the PC1/3 Promoter Requires Nhlh2 and STAT3
Activity of the WT PC1/3-luc reporter transfected in N29–2 cells in the absence (white bars) or presence (black bars) of leptin. Cells were transfected with PC1/3-luc reporter alone, or in combination with Nhlh2 alone, STAT3 alone, or with both Nhlh2 and STAT3 as indicated. Luciferase activity was measured and normalized to the expression of β-gal-encoding plasmid. Normalized activity is presented relative to the values obtained in cells transfected with PC1/3-luc alone ± se; *, P < 0.05.