Table 1.

Oligonucleotides Used in Quantitative PCR, Subcloning, Mutagenesis, ChIP, and EMSA Experiments

Oligonucleotide Sequence (5′–3′)	Purpose
AGTTGGAGGCATAAGGCTG	qRT-PCR, murine PC1/3 mRNA, forward
GCCTTCTGGGCTAGTCTG	qRT-PCR, murine PC1/3 mRNA, reverse
AACAGATCTCCTAGATTGTGTCTGTTTTATAACAAATGC	Subcloning, murine PC1/3 promoter, forward
TCCAAGCTTCAAAGAGAACCGCCTCATTTGAATATAACT	Subcloning, murine PC1/3 promoter, reverse
GTCTGTTTTATAACAAATGCTGAT	ChIP assay, murine PC1/3 promoter, forward
CGCCTCATTTGAATATAACTACCA	ChIP assay, murine PC1/3 promoter, reverse
GTCTGTTTTATAACAAATGCTGAT	EMSA, murine PC1/3 site 1, sense
ATCAGCATTTCTTATAAAACAGAC	EMSA, murine PC1/3 site 1, antisense
TGGTAGTTATTCAAATGAGGC	EMSA, murine PC1/3 site 2, sense
CGCCTCATTTGAATATAACTACCA	EMSA, murine PC1/3 site 2, antisense
GGGCCCTCATTTTCATGTGGGGCC	EMSA, murine necdin, sense
CCCCCAGGCCCCACATGAAAATGA	EMSA, murine necdin, antisense
GGATGGGTGCGTGGGGCC	EMSA, mutant necdin, sense
GGCCCCACGCACCCATCC	EMSA, mutant necdin, antisense
GATCTCCTAGATTGTGTCTGTTTTATAATGAATGCTGATACTCAAGAACCAT	Murine PC1/3ΔE1, forward
ATGGTTCTTGAGTATCAGCATTCATTATAAAACAGACACAATCTAGGAGATC	Murine PC1/3ΔE1, reverse
GCCTTGGGATCTTGGTAGTTATATTCAACCGAGGCGGTTCTCTTT	Murine PC1/3ΔE2, forward
AAAGAGAACCGCCTCGGTTGAATATAACTACCAAGATCCCAAGGC	Murine PC1/3ΔE2, reverse
CTCGAGATCTCCTAGATTGTGTCTGTGCCATAACAAATGCTGATACTCAAGAACC	Murine PC1/3ΔS12, forward
GGTTCTTGAGTATCAGCATTTGTTATGGCACAGACACAATCTAGGAGATCTCGAG	Murine PC1/3ΔS12, reverse
TTCCTGCCTTGGGATCTTGGTATCCATATTCAAATGAGGCGGTTCTC	Murine PC1/3ΔS12, forward
GAGAACCGCCTCATTTGAATATGGATACCAAGATCCCAAGGCAGGAA	Murine PC1/3ΔS12, reverse
CGGGCTCGAGATCTCCTAGATTGTGTCTGTGCCATAATGAATGCTGATACTCAAGAACCATTATAAGAA	Murine PC1/3Δ, using PC1/3ΔE12, forward
TTCTTATAATGGTTCTTGAGTATCAGCATTCATTATGGCACAGACACAATCTAGGAGATCTCGAGCCCG	Murine PC1/3Δ, using PC1/3ΔE12, reverse
CTTGGGATCTTGGTATCCATATTCAACCGAGGCGGTTCTCTT	Murine PC1/3Δ, using PC1/3ΔE12, forward
AAGAGAACCGCCTCGGTTGAATATGGATACCAAGATCCCAAG	Murine PC1/3Δ, using PC1/3ΔE12, reverse