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. Author manuscript; available in PMC: 2008 Jun 6.
Published in final edited form as: J Interferon Cytokine Res. 2000 Dec;20(12):1065–1070. doi: 10.1089/107999000750053726

FIG. 2.

FIG. 2

Stat signaling specificity of chimeric EpoR. 32D cells expressing R129C, R129C/343-S3, R129C/401-S3, or R129C/343/401-S3 were cultured in RPMI for 4 h, then stimulated with 20 U/ml Epo for 8 min at 37°C. Stat3 (lanes 1, 3, 5, 7) and Stat5 (lanes 2, 4, 6, 8) were immunoprecipitated from detergent cell extracts, and proteins were analyzed by antiphosphotyrosine immunoblots. A digital image of the autora-diogram is shown.