Table 1.
Virus (dilution) | Epo-independent CFU-E |
---|---|
None | 5 ± 3 (0) |
SFF-R129C | 82 ± 11 (77) |
SFF-R129C (1:3) | 20 ± 4 (15) |
SFF-R129C/343-S3 | 28 ± 1 (23) |
SFF-R129C/401-S3 | 40 ± 7 (35) |
SFF-R129C/343/401-S3 | 39 ± 7 (34) |
Fetal liver cells from day 13 mouse embryos were infected with retroviruses carrying constitutively active EpoR (as indicated). To adjust for differences in viral titer, a 1:3 dilution of SFF-R129C was also used. Erythroid colony-forming assays were performed as described in Materials and Methods. Mean colony numbers from triplicate cultures are shown for a representative experiment.