Skip to main content
. 2008 Jun 10;6(6):e140. doi: 10.1371/journal.pbio.0060140

Figure 1. Splice Variants of the CDC14B Parental Gene and Subcellular Localization of Encoded Proteins.

Figure 1

(A) Exon and protein structures of CDC14B splice variants. Color code of coding exons (exons not drawn to scale): exons shared by all variants are colored in dark blue; exons colored in yellow, light blue, green, and red are specific to transcripts CDC14B1, CDC14B2, CDC14B3, and CDC14Bpar, respectively, as indicated by the schematic representation of splicing patterns. The basic protein structure and sequence encoded by the different splice variants is indicated (only the protein sequence that differs between the splice variants is color-coded to indicate the relationship with the alternative splicing patterns of the coding exons). The retroduplication event of the CDC14Bpar transcript that gave rise to the CDC14B retrogene (CDC14Bretro) is illustrated.

(B–E) COS7-cells (from African green monkeys—no endogenous CDC14Bretro gene) transfected with CDC14B-DsRed fusion constructs (i.e., CDC14B signals in red) are shown. Microtubules were stained using an anti-beta tubulin antibody (green) and the nucleus was stained using DAPI (blue). Merged images: CDC14B1 (nucleoli) and −2 (nuclear speckles) localize to the nucleus (violet) and CDC14B3 and CDC14Bpar co-localize with microtubules (yellow). We obtained very similar results when using HeLa or LN229 (glioblastoma) cell lines (both carrying an endogenous CDC14Bretro gene, Figure S1). Thus, there appear to be no cell line–specific effects with respect to the observed subcellular localization patterns. Scale bars (white) = 10 μm.