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. 2008 Jun 10;6(6):e140. doi: 10.1371/journal.pbio.0060140

Figure 7. Microtubule Stabilization Capacity of CDC14Bretro and Parental CDC14B Variants.

Figure 7

Cells transfected with CDC14Bretro/CDC14B were treated with the depolymerizing agent nocodazole (see Materials and Methods for details). All experiments were repeated five times. Representative images of COS7 cells treated with nocodazole 24 h after transfection with human CDC14Bretro and human CDC14Bpar (fused to DsRed), in which microtubules (labeled in green) are stabilized (right image, yellow/green filaments; yellow indicates CDC14Bpar-microtubule co-localization) or degraded (left image) are shown.

Statistical analysis reveals significant stabilization differences between the different CDC14B proteins (one-way ANOVA, p < 10−4). Relevant pairwise comparisons of proteins that show significant stabilization differences (p < 0.01, Tukey's Post Hoc test): (i) CDC14Bretro from humans, chimpanzee, gorilla, node A versus CDC14Bretro from orangutan, gibbon, node B, CDC14Bpar, and CDC14B3; (ii) CDC14B1 and CDC14B2 versus CDC14Bpar/CDC14B3; (iii) node B versus BAB/ABA hybrids; (iv) BAB versus ABA; (v) CDC14Bretro from orangutan and gibbon versus CDC14Bretro from node B and CDC14Bpar. Scale bars = 10 μm.