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. 2008 Jun 10;6(6):e141. doi: 10.1371/journal.pbio.0060141

Figure 6. TTG1 Movement between Cell Layers.

Figure 6

(A) ppcA1:GFP–YFP in a young leaf. GFP/YFP-specific fluorescence is found in the subepidermal but not in the epidermal cell layer.

(B) ppcA1:GFP–YFP in an old leaf. Fluorescence is restricted to the subepidermal cell layers.

(C) ppcA1:YFP in a young leaf. YFP is found in all cells.

(D) ppcA1:YFP in an old leaf. Subepidermal expressed YFP is occasionally found in the epidermis (arrow). Trichomes show little or no fluorescence as shown in this picture.

(E) ppcA1:TTG1–YFP in a young leaf. Fluorescence is found in all cell layers.

(F) ppcA1:TTG1–YFP in an older leaf. In the epidermis, only trichomes exhibit fluorescence. Inset shows epidermis at higher magnification. Green, specific YFP fluorescence; red, chlorophyll fluorescence.