Figure 3.

Interaction of in vitro-translated DNA ligase I polypeptides with GST-PCNA. DNA ligase I and the deleted versions of DNA ligase I indicated were synthesized by coupled in vitro transcription and translation and then incubated with either GST-PCNA or GST beads as described in Materials and Methods. After separation by SDS/PAGE, labeled polypeptides were detected by fluorography. The positions of labeled molecular mass standards (Amersham) are indicated on the left. Lane 1, in vitro-translated polypeptide (one-tenth of input into binding reaction). Labeled polypeptides bound to: lane 2, GST-PCNA beads and lane 3, GST beads.