Figure 2.

Autoradiogram of denaturing 12% polyacrylamide gel comparing extent of nicking by mouse testes cell-free extracts of ɛA, Hx, and ɛC in control samples (lanes 1–3), wt mice (lanes 4–6) and ko mice (lanes 7–9). The positions of 5′ cleavage are shown on the far right, as indicated by arrows. The oligomers were allowed to react with 30 μg of protein for 1 h at 37°C in a standard nicking assay.