FIG. 4.

Degradation of 1 g liter⁻¹ CGP in 10 ml BM by axenic cultures of P. alcaligenes strain DIP1 in Hungate tubes cultivated anaerobically at 30°C under shaking conditions. a, tube containing 0.5 g liter⁻¹ yeast extract and 10 mM sodium nitrate and showing the fastest CGP degradation by P. alcaligenes strain DIP1 with visual disappearance of CGP and the lowest OD_{578 nm} after one and four incubation days, respectively; b, tube containing 0.5 g liter⁻¹ yeast extract and no sodium nitrate and showing the visual disappearance of CGP and the lowest OD_{578 nm} after four and five incubation days, respectively; c, tube containing 10 mM sodium nitrate and no yeast extract and showing the visual disappearance of CGP and the lowest OD_{578 nm} after 12 and 19 incubation days, respectively; d, tube containing neither nitrate nor yeast extract (negative control).