FIG. 4.

The average levels of hydropathy of (a) individual CDR-H3 sequences or (b) the combination of CDR-H3 sequences and natural toxin loops found in Crybodies with uninterrupted open reading frames. Crybodies that formed or failed to form crystalline inclusions when expressed in sporulating B. thuringiensis IPS 78/11 are shown in green and red, respectively. Correctly assembled Crybodies or those with framework mutations are represented by diamonds and plus signs, respectively. The average levels of hydropathy of the natural toxin loops are labeled and shown by gray horizontal lines. From left to right, the identity of each construct is as follows: for the cc1 library, 8, 11, 13, 14, 15, 16, 21, and 25; for the cc2 library, 2, 4, 5, 6, 9, 20, 22, 26, and 30; for the cc3 library, 2, 6, 9, 10, 16, 17, 20, and 24; for the cc12 library, 4, 11, 12, 13, 15, 17, 24, 25, 26, and 28; for the cc13 library, 3, 4, 9, 11, 12, 15, 16, 20, and 22; for the cc23 library, 1, 2, 3, 8, 14, 21, 23, and 24; and for the cc123 library, 1, 2, 5, 7, 8, 9, and 10.