FIG. 7.

The relationship between the presence of arginine or lysine residues in CDR-H3 inserts and Crybody sensitivity to internal trypsin cleavage. (a) The number of arginine or lysine residues present in CDR-H3 inserts of Crybodies susceptible (red) or resistant (green) to internal trypsin cleavage. Correctly assembled Crybodies are represented by diamonds, whereas Crybodies with framework mutations are shown by plus signs. (b) The percentages of Crybodies resistant to internal trypsin cleavage, grouped by the number of arginine or lysine residues present in their CDR-H3 sequence. The ratio of resistant to total Crybodies is shown in parentheses at each data point. Only correctly assembled Crybodies were considered in this analysis.