FIG. 8.

Crybody toxicity toward M. sexta neonates. Strains of B. thuringiensis IPS 78/11 expressing wild-type Cry1Aa13 (+) or a Crybody (as indicated) were cultured in CCY medium supplemented with 6 μg/ml chloramphenicol until complete sporulation was observed by phase contrast microscopy (48 to 72 h). Twenty microliters of each culture was applied to the surface of M. sexta diet in 48-well plates. Control wells (−) contained only diet. Neonates were added and plates incubated for 5 days, at which point mortality was assessed. Crybodies from libraries cc1, cc2, and cc3 were tested in three independent trials, six insects per trial. Crybodies from libraries cc12 and cc23 were tested twice, six insects per trial. Asterisks indicate Crybodies with framework mutations.