TABLE 1.

Percentages of correctly assembled Crybody library variants, as determined by sequence analysis

Library	No. of variants	% of correctly assembled Crybody library variants
		5′ junctiona	CDR-H3 insertb	3′ junctionc	Frameworkd	Assemblye
cc1	9	100	89	100	67	67
cc2	10	100	100	100	90	90
cc3	9	100	89	100	100	89
cc12	12	100	92	100	67	58
cc13	11	100	91	100	73	73
cc23	12	100	92	100	67	67
cc123	10	100	90	90	80	60
Total	73	100	92	99	77	71

^aThe sequence was GGT at the junction.

^bThe length of the insert was evenly divisible by 3.

^cThe sequence was TGG or TGC at the junction.

^dSixty base pairs of sequence upstream or downstream of the insertion were free of point mutations, insertions, and deletions.

^eThe assembly was error free over the entire sequence.