TABLE 1.
Flow cytometric analysis of M. tuberculosis carrying the glbN transcriptional fusion
| Time postinfection (h) | Fluorescence intensity (mean ± SE) of M. tuberculosis with pSCNPa
|
|
|---|---|---|
| Grown in vitro | Grown in vivo | |
| 4 | 49.73 ± 0.35 | 45.57 ± 0.32 |
| 12 | 48.30 ± 0.31 | 48.46 ± 0.25 |
| 24 | 48.95 ± 0.28 | 49.82 ± 0.23 |
| 48 | 50.00 ± 0.41 | 61.44 ± 0.52 |
a
The M. tuberculosis cells carried the glbN transcriptional fusion (pSCNP) and were harvested from shake flasks (grown in vitro) or infected THP-1 macrophages (grown in vivo). Three independent experiments were carried out, each in duplicate. The data are from one representative experiment.