TABLE 2.
Flow cytometric analysis of M. tuberculosis carrying the glbO transcriptional fusion
| Time postinfection (h) | Fluorescence intensity (mean ± SE) of M. tuberculosis with pSCOPa
|
|
|---|---|---|
| Grown in vitro | Grown in vivo | |
| 4 | 23.41 ± 0.20 | 25.00 ± 0.32 |
| 12 | 29.82 ± 0.13 | 29.79 ± 0.16 |
| 24 | 28.90 ± 0.22 | 29.84 ± 0.03 |
| 48 | 23.41 ± 0.27 | 42.41 ± 0.44 |
a
The M. tuberculosis cells carried the glbO transcriptional fusion (pSCOP) and were harvested from shake flasks (grown in vitro) or infected THP-1 macrophages (grown in vivo).Three independent experiments were carried out, each in duplicate. The data are from one representative experiment.