TABLE 2.

Cellobiohydrolase (cbhI) gene fragments recovered from sporocarp DNAs of six fungal species and best BLAST P matches of putative CBHI protein fragments recovered after removal of introns and translation^a

Species (putative gene)	Length of amplicon (bp)	Intron				BLAST P match (% identity)
		Insertion position(s)	Length(s) (bp)	Splice site(s)
				5′	3′
Clitocybe nuda cbhI570	570	418	58	GTAAGC	CAG	Agaricus bisporus cellobiohydrolase, CAA90422 (82.4)
Clitocybe nuda cbhI580	580	418	68	GTGAGT	TAG	Volvariella volvacea cbhI-II, AAT64007 (81.2)
Clitocybe nuda cbhI571	571	418	59	GTAAGT	CAG	Volvariella volvacea cbhI-II, AAT64007 (76.5)
Clitocybe gibba cbhI562	562	418	49	GTGAGA	CAG	Volvariella volvacea cbhI-II, AAT64007 (81.2)
Clitocybe gibba cbhI564	564	418	51	GTCAGC	TAG	Volvariella volvacea cbhI-II, AAT64007 (81.2)
Clitocybe gibba cbhI566	566	418	53	GTCAGT	TAG	Volvariella volvacea cbhI-I, AAT64006 (82)
Clitocybe c.f. gibba cbhI562	562	418	49	GTGAGA	CAG	Volvariella volvacea cbhI-II, AAT64007 (81.2)
Clitocybe c.f. gibba cbhI564	564	418	51	GTCAGC	TAG	Volvariella volvacea cbhI-II, AAT64007 (82.3)
Clitocybe c.f. gibba cbhI566	566	418	53	GTCAGT	TAG	Volvariella volvacea cbhI-I, AAT64006 (81.2)
Clitopilus prunulus cbhI	564	418	51	GTAAGC	CAG	Volvariella volvacea cbhI-II, AAT64007 (76.5)
Chlorophylum molybdites cbhI568	568	418	55	GTGAGT	TAG	Agaricus bisporus exocellobiohydrolase, CAA90422 (82.9)
Chlorophylum molybdites cbhI567	567	418	55	GTAAGC	TAG	Volvariella volvacea cbhI-II, AAT64007 (80.0)
Sarcoscypha occidentalis cbhI	615	146, 442	42, 46	GTATGA, GTTCGA	AAG, CAG	Pleurotus ostreatus sp. “Florida” 1,4-β-cellobiosidase (76.7)
Xylaria polymorpha cbhI	518					Sclerotinia sclerotiorum hypothetical GH7 protein (75.6)

^aIntron positions were inferred by alignment against known cbhI mRNA sequences. Gene fragments were amplified by PCR with the fungcbh primer set.