TABLE 2.
Species (putative gene) | Length of amplicon (bp) | Intron
|
BLAST P match (% identity) | |||
---|---|---|---|---|---|---|
Insertion position(s) | Length(s) (bp) | Splice site(s)
|
||||
5′ | 3′ | |||||
Clitocybe nuda cbhI570 | 570 | 418 | 58 | GTAAGC | CAG | Agaricus bisporus cellobiohydrolase, CAA90422 (82.4) |
Clitocybe nuda cbhI580 | 580 | 418 | 68 | GTGAGT | TAG | Volvariella volvacea cbhI-II, AAT64007 (81.2) |
Clitocybe nuda cbhI571 | 571 | 418 | 59 | GTAAGT | CAG | Volvariella volvacea cbhI-II, AAT64007 (76.5) |
Clitocybe gibba cbhI562 | 562 | 418 | 49 | GTGAGA | CAG | Volvariella volvacea cbhI-II, AAT64007 (81.2) |
Clitocybe gibba cbhI564 | 564 | 418 | 51 | GTCAGC | TAG | Volvariella volvacea cbhI-II, AAT64007 (81.2) |
Clitocybe gibba cbhI566 | 566 | 418 | 53 | GTCAGT | TAG | Volvariella volvacea cbhI-I, AAT64006 (82) |
Clitocybe c.f. gibba cbhI562 | 562 | 418 | 49 | GTGAGA | CAG | Volvariella volvacea cbhI-II, AAT64007 (81.2) |
Clitocybe c.f. gibba cbhI564 | 564 | 418 | 51 | GTCAGC | TAG | Volvariella volvacea cbhI-II, AAT64007 (82.3) |
Clitocybe c.f. gibba cbhI566 | 566 | 418 | 53 | GTCAGT | TAG | Volvariella volvacea cbhI-I, AAT64006 (81.2) |
Clitopilus prunulus cbhI | 564 | 418 | 51 | GTAAGC | CAG | Volvariella volvacea cbhI-II, AAT64007 (76.5) |
Chlorophylum molybdites cbhI568 | 568 | 418 | 55 | GTGAGT | TAG | Agaricus bisporus exocellobiohydrolase, CAA90422 (82.9) |
Chlorophylum molybdites cbhI567 | 567 | 418 | 55 | GTAAGC | TAG | Volvariella volvacea cbhI-II, AAT64007 (80.0) |
Sarcoscypha occidentalis cbhI | 615 | 146, 442 | 42, 46 | GTATGA, GTTCGA | AAG, CAG | Pleurotus ostreatus sp. “Florida” 1,4-β-cellobiosidase (76.7) |
Xylaria polymorpha cbhI | 518 | Sclerotinia sclerotiorum hypothetical GH7 protein (75.6) |
Intron positions were inferred by alignment against known cbhI mRNA sequences. Gene fragments were amplified by PCR with the fungcbh primer set.