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. 2008 Apr 4;74(11):3559–3572. doi: 10.1128/AEM.02722-07

FIG. 2.

FIG. 2.

Unique hydrogenase and urease gene clusters of N. multiformis. (A) The hydrogenase-encoding gene cluster from Nmul_A1662 to Nmul_A1677 (Nmul_A1662-1677; genes are abbreviated by their last four numbers) and its putative products and functions are as follows: Nmul_A1662, exonuclease metallo-beta-lactamase RNA processing enzyme; Nmul_A1663, response regulator GAF:metal-dependent phosphohydrolase HD domain (sigma-54 related); Nmul_A1664 (hypC), chaperone; Nmul_A1665 (hypA), hydrogenase expression and synthesis; Nmul_A1666 (hypB), hydrogenase accessory protein; Nmul_A1667 (hypD), hydrogenase formation; Nmul_A1668 (hypF), [NiFe] hydrogenase maturation protein; Nmul_A1669 (hypE), hydrogenase expression/formation protein; Nmul_A1670 to Nmul_A1673, four subunits of hydrogen dehydrogenase (EC 1.12.1.2) (Nmul_A1670 [hoxF], NAD-reducing hydrogenase [diaphorase] and 51-kDa alpha subunit; Nmul_A1671 [hoxU] ferredoxin gamma subunit Nmul_A1672 [hoxY], coenzyme F420-reducing delta 20-kDa small-subunit; Nmul_A1673 [hoxH], large-subunit); Nmul_A1674, unknown with N-terminal signal sequence and C-terminal PEP motif; Nmul_A1675 (hoxW), hydrogenase maturation peptidase; Nmul_A1676, GCN5-related acetyltransferase coenzyme A-binding transcriptional activation; Nmul_A1677, unknown function with N-terminal signal sequence and C-terminal PEP motif. (B) Urease-encoding cluster and regulatory regions, including the genes from Nmul_A1233 to Nmul_A1246 (Nmul_A1233-1246; genes are abbreviated by their last four numbers), and their products or functions are as follows: Nmul_A1233, TonB-dependent receptor; Nmul_A1234, putative transcriptional regulator and CopG family of nickel-responsive regulators; Nmul_A1235, two-component transcriptional regulator LuxR (regulatory protein, response regulator receiver containing a CheY-like receiver domain and a helix-turn-helix DNA-binding domain); Nmul_A1236, periplasmic sensor signal transduction histidine kinase; Nmul_A1237, FmdC precursor and urea-responsive OM porin; Nmul_A1238, accessory protein UreD; Nmul_A1239 to Nmul_A1241, structural subunits UreA, UreB, and UreC, respectively; Nmul_A1242 to Nmul_A1244, accessory proteins UreE, UreF, and UreG, respectively; Nmul_A1245, urea transporter; Nmul_A1246, transcriptional regulator, putative ATPase, winged helix family.